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Figure Lengend Snippet: In late S/G2 following DNA replication (and in conjunction with the ATM‐mediated DNA damage response at functional telomeres), RNF8 puts K63‐Ub chains on tankyrase 1, promoting its association with telomeres and sister telomere resolution. Following nuclear envelope breakdown, ABRO1 (and BRCC36) gains access to K63‐Ub TNKS1 and removes the chains. ABRO1 remains in the nucleus through G1 keeping the K63‐Ub chains off tankyrase 1 and preventing premature resolution of telomere cohesion in S phase. ABRO1 is exported from the nucleus in S phase to permit K63‐ubiquitination of tankyrase 1. C, cytoplasm; N, nucleus.

Article Snippet: Immunoblots were incubated separately with the following primary antibodies: rabbit anti‐tankyrase 1 762 (1 μg/ml) (Scherthan et al , 2000 ); rabbit anti‐Flag (1 μg/ml) (Sigma‐Aldrich, F7425); rabbit c‐Myc (0.2 μg/ml) (Santa Cruz, sc‐789); mouse anti‐α‐tubulin ascites (1:10,000) (Sigma‐Aldrich, T5768); mouse cyclin B1 (0.2 μg/ml) (Santa Cruz, sc‐245); rabbit phospho‐H3 (1 μg/ml) (Millipore, 06‐570); rabbit HA (0.5 μg/ml) (Abcam, ab9110); rabbit anti‐RNF8 (1 μg/ml) (Abcam, ab4183); rabbit lamin B serum (1:1,000) (Chaudhary & Courvalin, 1993 ); rabbit anti‐BRCC36 (0.5 μg/ml) (Bethyl, A302‐517A); rabbit anti‐ABRO1 (1 μg/ml) (Bethyl, A301‐255A); and rabbit anti‐ubiquitin Lys63‐specific clone Apu3 (1:1,000) (Millipore, 05‐1308), followed by horseradish peroxidase‐conjugated donkey anti‐rabbit or anti‐mouse IgG (Amersham) (1:2,500).

Techniques: Functional Assay

Journal: Molecular cell

Article Title: p300-Mediated Lysine 2-Hydroxyisobutyrylation Regulates Glycolysis

doi: 10.1016/j.molcel.2018.04.011

Figure Lengend Snippet: Key Resources Table

Article Snippet: Rabbit polyclonal anti-PFKM antibody , Bethyl Laboratories , Cat# A304-255A.

Techniques: Recombinant, shRNA, Clone Assay, Synthesized, Activity Assay, Mutagenesis, Mass Spectrometry, Western Blot, Sequencing, Software